Journal: Physiological Research

Article Title: Hydrogen Sulfide Alleviates Lipopolysaccharide-Induced Myocardial Injury Through TLR4-NLRP3 Pathway

doi: 10.33549/physiolres.934928

Figure Lengend Snippet: Exogenous H2S amelio-rates myocardial tissue injury. (A) Representative M-mode images from Control, LPS, and LPS+NaHS groups. (B) Represen-tative HE-stained left ventricular sections (scale bar = 50 μm). (C) The changes of left ventri-cular ejection fraction (LVEF). (D) Pathological score of the HE-stained left ventricular sec-tions. (E) H2S levels in the SIMD mice model plasma. (F) cTnI levels in the SIMD mice model plasma. (G) TNF-α levels in the SIMD mice model plasma. (H) IL-1β levels in the SIMD mice model plasma. (I–J) Represen-tative Western blots and quan-tification of TLR4 and NLRP3 protein expression in heart tissues. β-actin was used as the internal control. n=8 in every mice model group. n=5 in every Western blot group. Results are means ± SEM. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. P<0.05 was considered significant.

Article Snippet: To evaluate left ventricular function, mouse two-dimensional echocardiography was performed using a VisualSonics Vevo 770 platform.

Techniques: Control, Staining, Clinical Proteomics, Western Blot, Expressing

Journal: Physiological Research

Article Title: Hydrogen Sulfide Alleviates Lipopolysaccharide-Induced Myocardial Injury Through TLR4-NLRP3 Pathway

doi: 10.33549/physiolres.934928

Figure Lengend Snippet: NLRP3 inhibitor, MCC950, attenuates LPS-induced myocardial injury. (A) Representative M-mode images from LPS, LPS+MCC950 and LPS+NaHS+MCC950 groups (B) Representative HE-stained left ventricular sections (scale bar = 50 μm). (C) The changes of left ventricular ejection fraction (LVEF). (D) Pathological score of the HE-stained left ventricular sections. (E) cTnI levels in the SIMD mice model plasma. (F) TNF-α levels in the SIMD mice model plasma. (G) IL-1β levels in the SIMD mice model plasma. n=8 in each group. Results are means ± SEM. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. P<0.05 was considered significant.

Article Snippet: To evaluate left ventricular function, mouse two-dimensional echocardiography was performed using a VisualSonics Vevo 770 platform.

Techniques: Staining, Clinical Proteomics

Journal: Physiological Research

Article Title: Hydrogen Sulfide Alleviates Lipopolysaccharide-Induced Myocardial Injury Through TLR4-NLRP3 Pathway

doi: 10.33549/physiolres.934928

Figure Lengend Snippet: NaHS exerts protective effects mainly through the TLR4-NLRP3 signaling axis (A) Representative M-mode images from WT, WT+LPS, TLR4−/−+LPS, TLR4−/−+LPS+NaHS groups (B) Representative HE-stained left ventricular sections (scale bar = 50 μm). (C) The changes of left ventricular ejection fraction (LVEF). (D) Pathological score of the HE-stained left ventricular sections. (E) cTnI levels in the SIMD mice model plasma. (F) TNF-α levels in the SIMD mice model plasma. (G) IL-1β levels in the SIMD mice model plasma. n=8 in each group. Results are means ± SEM. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. P<0.05 was considered significant.

Article Snippet: To evaluate left ventricular function, mouse two-dimensional echocardiography was performed using a VisualSonics Vevo 770 platform.

Techniques: Staining, Clinical Proteomics

Journal: bioRxiv

Article Title: A Transient Increase in Cardiomyocyte Protein O-GlcNAcylation Enhances Susceptibility to Pressure Overload-Induced Cardiac Remodeling

doi: 10.1101/2025.06.04.657956

Figure Lengend Snippet: Schematic showing the experimental timeline and procedures for the dnOGAh ON/OFF-TAC paradigm used in this study—from induction of dnOGA transgene via DOX, switching the transgene “off” via removal of DOX, and TAC procedure, to subsequent echocardiography and tissue harvesting for downstream analyses.

Article Snippet: At the prespecified timepoints shown in the experimental schema ( ) after 8 weeks of TAC/Sham, transthoracic echocardiography was performed on all mice in the study, as previously described., In brief, the mice were anesthetized via 2% isoflurane induction chamber inhalation then placed in a supine position on a heated echocardiography platform (Vevo integrated rail system, VisualSonics) set at 37°C to maintain body temperature; sedation was maintained via nose cone inhalation of 1.0-1.5% isoflurane which allowed for heart rate maintenance of ∼400 beats / min. Fur from the chest was removed via topical depilatory cream, and then the mice were then placed in a left-lateral-decubitus position.

Techniques:

Journal: bioRxiv

Article Title: A Transient Increase in Cardiomyocyte Protein O-GlcNAcylation Enhances Susceptibility to Pressure Overload-Induced Cardiac Remodeling

doi: 10.1101/2025.06.04.657956

Figure Lengend Snippet: A) Normalized ratio of wet-dry lung weight to TL for Con-TAC and ON/OFF-TAC mice compared to age-matched Sham controls (Con-Sham and ON/OFF-Sham) ( n ≥ 29). B) Normalized ratio of wet-dry lung weight to TL for Con-TAC and ON/OFF-TAC mice compared to age-matched Sham controls (Con-Sham and ON/OFF-Sham) split by sex. C-G) Cardiac function measures via echocardiography split by sex for: cardiac systolic function as measured by LV ejection fraction from M-mode LV traces ( C ), stroke volume from M-mode LV traces ( D ), left ventricular internal dimension at diastole (LVIDd) ( E ), cardiac wall thickening as measured by LV posterior wall thickness at diastole (LVPWd) ( F ), and inter-ventricular septum thickness at diastole (IVSd) ( G ). Statistical significance was based on two-way ANOVA followed by Tukey’s multiple comparisons test (* p < 0.05, ** p < 0.01, *** p < 0.005). All calculated and analysis data are mean ± S.E.M.

Article Snippet: At the prespecified timepoints shown in the experimental schema ( ) after 8 weeks of TAC/Sham, transthoracic echocardiography was performed on all mice in the study, as previously described., In brief, the mice were anesthetized via 2% isoflurane induction chamber inhalation then placed in a supine position on a heated echocardiography platform (Vevo integrated rail system, VisualSonics) set at 37°C to maintain body temperature; sedation was maintained via nose cone inhalation of 1.0-1.5% isoflurane which allowed for heart rate maintenance of ∼400 beats / min. Fur from the chest was removed via topical depilatory cream, and then the mice were then placed in a left-lateral-decubitus position.

Techniques:

Journal: Echo Research and Practice

Article Title: Transthoracic echocardiography is adequate for intraprocedural guidance of transcatheter aortic valve implantation

doi: 10.1530/ERP-17-0050

Figure Lengend Snippet: Transthoracic echocardiography during transcatheter aortic valve implantation procedure – excellent image quality. (A) Transthoracic parasternal short-axis view demonstrating severe calcific aortic valve stenosis. (B) Parasternal long-axis transthoracic view demonstrating normal depth, expansion and appearance immediately after transcatheter aortic valve implantation. Leaflets can be seen to move normally in these views. (C and D) Immediate post-implantation short-axis parasternal view and apical 5-chamber view demonstrating mild paravalvular regurgitation. In the short-axis view, normal Doppler interference from the transcatheter stent is visualized and the paravalvular regurgitation is turbulent. (E and F) Using a 4-dimensional (4D) transthoracic echocardiography probe, real-time 4D images were acquired that demonstrated the normal appearance of transcatheter aortic valve implantation immediately after the procedure, an en-face view from the aorta (E), and an en-face view from the left ventricular outflow tract (F).

Article Snippet: As all echocardiography laboratories store echocardiograms on a single common platform (stored in Synapse Cardiovascular, Fujifilm; Stamford, CT, USA), we were able to query all patients who underwent TTE for intraprocedural guidance of TAVI, examining their pre-TAVI TTE, TTE-TAVI, 24-h post-TAVI TTE and 30-day follow-up TTE.

Techniques:

Journal: Echo Research and Practice

Article Title: Transthoracic echocardiography is adequate for intraprocedural guidance of transcatheter aortic valve implantation

doi: 10.1530/ERP-17-0050

Figure Lengend Snippet: Stacked bar graph of imaging characteristics of procedural echocardiography ( n = 278).

Article Snippet: As all echocardiography laboratories store echocardiograms on a single common platform (stored in Synapse Cardiovascular, Fujifilm; Stamford, CT, USA), we were able to query all patients who underwent TTE for intraprocedural guidance of TAVI, examining their pre-TAVI TTE, TTE-TAVI, 24-h post-TAVI TTE and 30-day follow-up TTE.

Techniques: Imaging

Journal: Echo Research and Practice

Article Title: Transthoracic echocardiography is adequate for intraprocedural guidance of transcatheter aortic valve implantation

doi: 10.1530/ERP-17-0050

Figure Lengend Snippet: Transthoracic echocardiography visualized intraprocedural pericardial effusion and tamponade – fair image quality. (A) Transthoracic parasternal long-axis view demonstrating severe calcific aortic stenosis and no pericardial effusion. (B) Immediately after transcatheter aortic valve implantation, pericardial effusion was visualized (orange arrow). (C) This pericardial effusion was redemonstrated on parasternal short-axis view and appeared larger; the left ventricle was demonstrated to be underfilled. This combined with hypotension required emergency endotracheal intubation and sternotomy. A pericardiocentesis was performed and a wire perforation was identified in the left ventricle and repaired. Despite fair image quality, trace paravalvular regurgitation was identified on TTE. (D and E) This same jet of paravalvular regurgitation was also noted on TOE images in short-axis and long-axis (F) esophageal views (green arrows).

Article Snippet: As all echocardiography laboratories store echocardiograms on a single common platform (stored in Synapse Cardiovascular, Fujifilm; Stamford, CT, USA), we were able to query all patients who underwent TTE for intraprocedural guidance of TAVI, examining their pre-TAVI TTE, TTE-TAVI, 24-h post-TAVI TTE and 30-day follow-up TTE.

Techniques:

Journal: Echo Research and Practice

Article Title: Transthoracic echocardiography is adequate for intraprocedural guidance of transcatheter aortic valve implantation

doi: 10.1530/ERP-17-0050

Figure Lengend Snippet: Assessment of paravalvular regurgitation in transthoracic TAVI ( n = 278 patients) at procedural echocardiography, 24-h echocardiography ( n = 278) and 1-month echocardiography ( n = 236). Echo = echocardiography; TAVI = transcatheter aortic valve implantation.

Article Snippet: As all echocardiography laboratories store echocardiograms on a single common platform (stored in Synapse Cardiovascular, Fujifilm; Stamford, CT, USA), we were able to query all patients who underwent TTE for intraprocedural guidance of TAVI, examining their pre-TAVI TTE, TTE-TAVI, 24-h post-TAVI TTE and 30-day follow-up TTE.

Techniques: